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Standard Operating Procedures (SOP's) for Duchenne Animal Models
Cardiac Protocols for Duchenne Animal Models
A workshop was organized by the National Heart, Lung, and Blood Institute (NHLBI), and sponsored by PPMD, in July 2014, in order to address Contemporary Cardiac Issues in Duchenne Muscular Dystrophy. This workshop resulted in 3 small groups; one of the groups was tasked to update and develop standard operating procedures (SOP’s) incorporating state of the art cardiac assessment and monitoring. Animal Model Small Group members included Dongsheng Duan (co-leader), Jill Rafael-Fortney (co-leader), Alison Blain, David Kass, Elizabeth McNally, Joseph Metzger, and Chris Spurney.
The standardization of operating procedures for cardiac research, and research involving cardiac assessment and monitoring in Duchenne animal models, hopes to improve the comparability of research studies performed in multiple laboratories. These SOP’s are not meant to be mandatory, but used as a point of reference amongst laboratories. They are meant to be "living documents," incorporating innovation and further improvement as they are implemented. Concerns/questions/suggestions should be directed to Kathi Kinnett at firstname.lastname@example.org.
All SOP's were created/updated June 2015, unless otherwise indicated.
1. General protocols
2. Functional assay protocols related to the rodent model
3. Functional assay protocols related to the large animal model
4. Rodent cardiac function reference values
5. Large animal cardiac function reference values
Non-Cardiac SOP’s for Duchenne Animal Models
Non-cardiac SOP’s for Duchenne animal models are available on the TREAT-NMD website. Two meetings were hosted with specialists from all over the world to discuss and create this collection of SOPs, in full collaboration with the Senator Paul D. Wellstone Muscular Dystrophy Cooperative Research Center at Children's National Medical Center in Washington DC and with the US National Institutes of Health (NIH)-Wellstone Muscular Dystrophy Cooperative Network, and with the generous support of the Foundation to Eradicate Duchenne Inc, the US National Institutes of Health and TREAT-NMD. These workshops were described in a meeting report published in Neuromuscular Disorders and available on the TREAT-NMD website.
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